HeLa/GFP-H2B细胞系的构建及对其有丝分裂进程的动态观察

摘要

Accurate chromosome segregation depends on the precise regulation of mitotic progression,including the timing of mitosis and proper regulation of spindle assembly checkpoint are getten.Though live-cell observation movement of chromosome,we could accurately quantify the procedure above.Here, though retroviral infection system we get stable expressed GFP-H2B HeLa cell line.Together with synchronizing cells to mitosis, a technique is established that observing mitosis though time-lapse confocal scanning laser microscopy.%准确的染色体分离依赖于有丝分裂过程的精确调控,包括有丝分裂的时间,及纺锤体检查点的正确调控等.通过动态观察有丝分裂染色体的运动可对上述研究进行精确定量.结果显示,利用逆转录病毒系统成功构建了稳定融合表达绿色荧光蛋白GFP-H2B的HeLa细胞系,结合细胞同步化方法,建立了一套利用活细胞荧光共聚焦显微镜观察HeLa细胞有丝分裂的实验体系.