生淀粉酶细菌菌株的筛选及其酶学性质研究

摘要

A strain QDO06 with higher activity of raw - starch glucoamylase was isolated and primitive identified as Staphylococcus intermediu by morphology and conventional biochemical identification. The enzymatic property revealed that optimum temperature was at 50 ℃ and pH 6.5. The enzyme had good thermal stability. The enzyme activity was activated by Na ＋ , K ＋ and Ca2＋ and restrained by Fe3＋ ,Zn2＋ and Cu2＋.%分离筛选到一株生淀粉酶酶活较高的菌株QD006，通过形态和常规生理生化鉴定，初步确定该菌为Staphylococcus Sintermedius。该菌产生的生淀粉酶最适温度为50℃，最适pH为6．5，该酶具有良好的热稳定性。Na＋、K＋、Ca2＋对该酶有激活作用，Fe3＋、Zn2＋和Cu2＋对该酶有抑制作用。