玫瑰茄花色苷纯化工艺优化研究

摘要

对玫瑰茄花色苷纯化工艺进行优化研究，通过对 X －5、DA －201、HPD －100、D101、AB －8五种大孔树脂进行筛选，得出最适用于玫瑰茄花色苷纯化的树脂为 HPD －100大孔树脂。通过单因素和正交实验对 HPD －100大孔树脂纯化条件进行优化，得出最佳吸附工艺条件是：样液浓度为1．5 mg／mL，上样流速为2 mL／min，pH 值为3．0，吸附率为91．988％；最佳解吸工艺条件是：洗脱液浓度为70％，洗脱流速为1．75 mL／min，洗脱液用量60 mL，解吸率为92．144％，纯化后的玫瑰茄红色素色价为38．5。通过纯化前后玫瑰茄红色素高效液相色谱图的比较可知：玫瑰茄红色素主要含有5种成分，纯化后的色素矢车菊花色苷的含量明显升高。%The purification of roselle anthocyanins was studied.The most suitable resin for roselle antho-cyanin purification was HPD -100 macroporous resin,which was obtained by screening five kinds of macroporous resins:X -5,DA -201,HPD -100,D101 and AB -8.The purification conditions for HPD -100 macroporous resin were optimized by single factor and orthogonal experiments,which were:the sample′s concentration 1.5 mg/mL,flow rate 2 mL/min,pH 3.The adsorption rate can reach 91.988%.The optimal desorption process conditions were eluent concentration 70%,elution velocity 1.75 mL/min,dosage of eluent 60 mL.The desorption rate can reach 92.144%.After the purification, the color value of roselle anthocyanins was 38.5.Compared before and after the purification,the chro-matograms by HPLC showed that roselle red pigment mainly contained five components.The cornflower anthocyanin content in the pigment increased significantly after being purified.