平阳霉素对阴茎癌组织c－myc及Ras－P21蛋白表达影响的研究

摘要

目的：探讨应用平阳霉素化疗对阴茎癌组织蛋白c－myc、Ras－P21表达的影响及意义。方法收集1995—2005年间阴茎鳞状细胞癌患者100例，按照术前是否进行化疗分为两组，其中化疗组50例，术前应用平阳霉素化疗7天，并在化疗后行阴茎部分切除术＋改良方法的腹股沟淋巴结清扫术；对照组50例，未进行化疗而直接行阴茎部分切除术改良方法的腹股沟淋巴结清扫术。应用免疫组化法（ SP）对两组的100例阴茎癌组织标本进行c－myc、Ras－P21蛋白产物检测。应用χ2检验对数据进行统计分析。结果化疗组50例阴茎癌标本中c－myc、Ras－P21表达阳性率分别为30％、27％。对照组50例阴茎癌标本中，c－myc、Ras－P21表达阳性率分别为52％、48％。经χ2检验，化疗组与对照组的c－myc、Ras－P21表达阳性率的差异具有统计学意义（P＜0．05）。结论应用平阳霉素化疗后阴茎癌组织中c－myc、Ras－P21蛋白的表达明显下降。%Objective To evaluate the influence and significance of Pingyangmycin chemotherapy on the c-myc and Ras-P21 protein expression in penile cancer .Methods A total of 100 penile squamous cell carci-noma cases was retrospectively studied and divided into two groups .Data were obtained from 1995 to 2005 .In the chemotherapy group ,50 cases of patients were selected to perform preoperative chemotherapy before surgery .The patients were treated by Pingyangmycin .After 7 times of medication ,partial excision of penis plus improved ingui-nal lymph node dissection was performed .In the control group ,50 cases of patients were selected for partial exci-sion of penis plus improved inguinal lymph node dissection directly without any pre -operative chemotherapy .All pathology specimens were detected of c -myc and Ras-P21 protein expression by immunohistochemical staining assay.Theχ2 test was used for the statistical analysis .Results In chemotherapy group,the positive expression rates of c-myc and Ras-P21 were 30%,27%,respectively.However,in control group,the positive expression rate of c-myc,Ras-P21 were 52%,48%,respectively.By theχ2 test,the expressive differences of c -myc,Ras-P21 positive expression rate between chemotherapy group and control group were all significant (P<0.05). Conclusion The protein expressions of c -myc and Ras-P21 is significantly decreased in the tissue of Pingy-angmycin chemotherapy of penile cancer .