温石棉及其代用纤维对鼠细胞钙激活蛋白43及B 淋巴细胞瘤-2表达的影响

摘要

Objective To investigate the effect of chrysotile and its substitute on expressions of CAP 43 and Bcl-2 in hamster cells and to explore the their possible carcinogenic mechanism .Methods Chinese hamster lung cells(V79 cells)were exposed to dif-ferent concentrations of chrysotile and its substitutes including chrysotile from Xinkang ,Sichuan(A),chrysotile from Southern Shanxi (B),glass fiber(C),ceramic fiber(D),wollastonite(E)and rockwool(F).At the same time,cells without treatment were used as nega-tive controls.The expressions of CAP43 and Bcl-2 in V79 cells were detected by using immunohistochemistry ,and compared by using statistical program SPSS21.0.Results The expressions of CAP43 and Bcl-2 in A and B were significantly up-regulated(P <0.05). As the concentration of powder increased ,immune staining OD value of CAP 43 and Bcl-2 were increased .Conclusion Chrysotile and its substitute can significantly influence the CAP 43 and Bcl-2 expressions in hamster cells .%目的：研究温石棉及其代用纤维对鼠细胞钙激活蛋白43（calciumactinated protein 43，CAP43）及B淋巴细胞瘤-2（B-cell lymghoma，Bcl-2）表达的影响，探讨温石棉及其代用品的致癌性机制。方法将不同浓度的温石棉及其代用品[四川新康温石棉（A）、陕西陕南温石棉（B）、玻璃纤维（C）、陶瓷纤维（D）、硅灰石（E）、岩棉（F），阴性对照组（G）]暴露于V79细胞，使用免疫组织化学法检测CAP43及Bcl-2的表达，并使用SPSS 21.0对表达结果进行对比分析。结果 A、B组的仓鼠肺细胞中CAP43及Bcl-2表达增强较为显著，与其他组比较，差异有统计学意义（ P＜0.05）；CAP 43及Bcl-2基因表达随着粉体浓度的增加，其OD值也呈上升趋势。结论温石棉及其代用纤维对于鼠细胞CAP43及Bcl-2表达有显著的增强作用。