乙型肝炎病毒前S2区缺失突变与肝癌相关性研究

摘要

Objective To explore the relationship between hepatocarcinoma and hepatitis B virus preS2 deletion mutation. Methods The gene of HBV pres fiom patients of hepatocarcinoma( HCC ) and asymptomatic HBV cariers ( ASC ) were amplified and sequenced. The sequences of all samples were analyzed using DNAMAN. Results 23.08％ ( 6/26 ) of HCC patients were confirmed as preS2 delete mutations and all of them were in the hot deletion regions in nt 3223 ～ nt 3268. The length of preS2 deletions ranged from 30 to 45 bp. PreS2 deletion is more frequently in HCC patients than ASC. Conclusion Deletion mutation of preS2 may be closely correlated with hepatocarcinogenesis. However, the mechanism of preS2 deletion mutation in oncogenesis needs to be further elucidated.%目的 研究肝细胞肝癌(hepatocarcinoma,HCC)患者乙型肝炎病毒(Hepatitis B virus,HBV)前S2区缺失突变情况.方法 应用PCR法扩增HBV 前S区基因,应用DNAMAN软件对所测基因进行分析.结果 23.08%(6/26)的肝癌患者发现存在preS2区的缺失突变,缺失位点为nt 3223～nt 3268,缺失长度为30～45 bp.肝癌患者缺失突变发生率显著高于HBV无症状携带者.结论 HBV preS2区的缺失突变可能与肝癌的发生密切相关,该突变的发生机制仍有待进一步研究.