五味子及其与丹参 灵芝 柴胡配伍对慢性肝损伤的影响

摘要

目的　观察五味子和五味子与丹参、柴胡、灵芝配伍对慢性肝损伤的治疗作用。方法　采用四氯化碳(CCl4)和D-氨基半乳糖(D-GlaN)多次注射造成两种慢性肝损伤模型，给予同一剂量的药物，测定血清谷丙转氨酶(ALT)、胆碱酯酶(CHE)、肝匀浆ALT、CHE、谷胱苷肽(GSH)、丙二醛(MDA)和碱性磷酸酶(ALP)，HE染色观察肝组织形态学。结果　五味子对CCl4慢性肝损伤所致的血清与匀浆ALT显著降低(P<0.01)，CHE升高(P<0.01)，ALP与MDA下降(P<0.01)。五味子+丹参对慢性肝损伤酶的作用与五味子相似，组织形态学结果与生化结果基本一致。五味子+柴胡与五味子+灵芝的作用弱于前者，但病理组织形态学结果不支持二者的治疗作用。对D-GlaN所致慢性肝损伤五味子能加强枯否细胞的吞噬功能，增加蛋白合成，消除MDA对细胞的损伤，改善细胞代谢。五味子+丹参的生化结果与组织形态学结果显示其治疗作用强于五味子，对纤维组织增生的抑制作用是其特点。五味子+灵芝虽有降ALT和升高CHE作用(P<0.05，P<0.01)，但对匀浆CHE无效，而组织形态学显示受损的肝细胞无改善。五味子+柴胡对酶的作用较差，组织形态学显示受损的肝细胞无明显改善。结论　五味子对两种慢性肝损伤有较好的治疗作用，五味子与丹参配伍的作用强于五味子，对纤维组织增生有明显抑制作用是其特点。%Aim To investigate the effect treatment of Schisandra chinensis(Turcz.) and Schisandra chinensis(Turcz.) compatible with Salvia miltiorrhiza Bge，Ganoderma lucidum and Bupleurum chinense DC resprectively on Chronic liver injuries in mice.Methods Both chronic liver injuries models were used by injection each the tetrachloromethane(CCl4) and D-Galactosamine for more times in mice. The mice were administrated the drug in the same dosage,then the activities of alanine aminotransferase(ALT) and cholinesterase were measured in plasma,Glutathione，alkalinephosphatase(ALP)，molondiadehyde(MDA) were measured as well,and the hepatic histomorphology were observed by HE staining.Result Schisandra chinensis(Turcz.) inhibited the elevated level of ALT dramaticlly,increased the actiuty of CHE and reduced ALP and MDA in the damaged mice induced by CCl4.However,it had no effects on GSH.The actions Schisandra chinensis(Turcz.) match with Salvia miltiorrhiza Bge were similar with those of Schisandra chinensis(Turcz.) and the result of bioanalysis was identical with histomorphology about the action on chronic hepatic trauma.The effects Schisandra chinensis(Turcz.) combination with Bupleurum chinense DC,Schisandra chinensis(Turcz.) and Ganoderma lucidum were weaker than those of the anterior.However,histomorphological results did not sustain it's cure action on chronic liver injuries.Schisandra chinensis(Turcz.) could strengthen the cupffer phagocytosis,increased protein synthesis and improved the cell metabolite on liver injury in mice induced by D-GlaN.The results of boianalysis and histomorphology dsuggested that the action of Schisandra chinensis(Turcz.) match with Salvia miltiorrhiza Bge was better than that of the Schisandra chinensis(Turcz.).Moreover,the action characteristic was inhibition the hyperplasia of fibrous tissue.Although the Schisandra chinensis(Turcz.) match with Ganoderma lucidum reduced ALT lever and elevated CHE, histomorphological results showed no improve ment on damage of liver cell in mice.Bupleurum chinense DC combination with Schisandra chinensis(Turcz.) have good enough effects in enzymatic activity,while histopathology shows them have not repair on lesion liver histogenesis.Conclusion Schisandra chinensis(Turcz.) has better cure action on both model chronic liver injuries.Schisandra chinensis(Turcz.) match with Salvia miltiorrhiza Bge is better than the Schisandra chinensis(Turcz.).Strong inhibition hyperplasia fibrosis tissue is the characteristic of them in mice chronic liver damage.