走马胎中的三萜皂苷类成分及其体外抗肿瘤活性研究

摘要

目的 研究走马胎根茎的三萜皂苷类成分及其体外抗肿瘤活性.方法 采用溶剂萃取和柱色谱方法分离纯化,根据理化性质和波谱学数据鉴定化合物结构.采用MTT法测试三萜皂苷化合物的抗肿瘤活性.结果 分离得到了7个三萜皂苷类化合物,包括3β-O- β-D-吡喃木糖基-(1→2)-β-D-吡喃葡萄糖基-(1→4)-α-L-吡喃阿拉伯糖基-西克拉敏A(1);3β-O- α-L-吡喃鼠李糖基-(1→3)-[β-D-吡喃木糖基-(1→2)]-β-D-吡喃葡萄糖基-(1→4-[β-D-吡喃葡萄糖-(1→2)]-α-L-吡喃阿拉伯糖基 -西克拉敏A(2);lysikoianoside(3);3β-O-β-L-毗喃鼠李糖基-(1→3)-[β-D-吡喃葡萄糖基-(1→3)-β-D-吡喃木糖基-(1→2)]-β-D-吡喃葡萄糖基-(1→4)-[B-D-吡喃葡萄糖基-(1→2)]-α-L-吡喃阿拉伯糖基-西克拉敏A(4),3β-O- β-L-吡喃鼠李糖基-(1→3)-[β-D-吡喃木糖基-(1→2)]-β-D-吡喃葡萄糖基-(1→4)-[β-D-6-O-乙酰氧基-吡喃葡萄糖基(1→2)]-α-L-吡喃阿拉伯糖基 -西克拉敏A(5),Ardisiacrispin A(6)和3β-o- α-rhamnopyranosyl-(1→3).[β-D-xylopyranose-(1→2)]-β-D-glucopyranosyl-(1→4)-αL-arabinopyranosyl -3β-hydroxy-13β,28-epoxy-oleanan-16-oxo-30-al(7).结论 化合物1和3为首次从该植物中分离得到,化合物7首次从该属植物中分离得到.其中化合物2对BCG-823,EJ和Hepg2细胞有较好的抑制活性(IC50分别为0.29,9.99和2.03μg/ml).化合物3对EJ细胞有选择性抑制作用(Ic50为7.20μg/m1),化合物7对Hepg2细胞有选择性抑制活性(IC50为8.53μg/ml).%Objective To study triterpenoid saponins and the antitumor activity of the 60％ ethanol extract of the rhizome of Ardisia gigantifolia Stapf. Methods The chemical components were isolated by solvent extraction and column chromatography and their structures were identified on the basis of physical-chemical constants and spectral data. The antitumor activities of the compounds were screened by MTT methods. Results Seven triterpenoid saponins were isolated and identified as 3β -0- ｛ β -D-xylopyranosyl- ( 1→2 ) - β -D-glucopyr-anosyl- ( 1 →4 ) - α -L-arabinopyranosyl ｝ -3 β -hydroxy-13β, 28-epoxy-16 β -hydroxy-30-al ( 1 ); cyclamiretin A 3 β -0- α -L-rhamnopyranosyl ( 1 → 3)-[ β -D-xylopyranosyl-( 1 →2) ]-β -D-glucopyranosyl-( 1 →4 )-[ β -D-glucopyranosyl ( 1 →2 ) ] - α -L-arabinopyranoside ( 2 ); lysikoianoside ( 3 ); cyclamiretin A 3 β -O- α -L-rhamnopyranosyl- ( 1→3 ) - [ β -D-glucopyranosyl- ( 1→3 ) - β -D-xylopyranosyl- ( 1→2 ) ] - β -D-glucopyranosyl- ( 1→4 ) - [ β -D-glucopyranosyl- ( 1→2) ]- α -L-arabinopyranoside (4), 3 β -0- o -L-rhamnopyranosyl-(l →3 ) -[ β -D-xylopyranosyl-( 1 →2 ) ] -β -D-galactopyranosyl- ( 1→4)- [ β -D-6-O-acetyl-glucopranosyl-(1→2) ]- α -L-arabinopyranoside -cyclamiretin A (5),Ardisiacri-dpin A ( 6 ) and 3 β-o-α-L-rhamnopyranosyl- ( 1→3 ) - [ β -D-xylopyranose- ( 1→2 ) ]- β -D-glucopyranosyl-( 1→4)- α -L-arabinopyranosyl -3β -hydroxy-13β ,28-epoxyoleanan-16-oxo-30-al(7). Conclusion Compound 1 and 3 were obtained from this plant and 7 from this genus for the first time. Among the compounds, No. 2 showed cytotoxic activity of 0.29, 9.99 and 2.03 μg/ml ( IC50 ) in BCG-823, EJ and Hepg2 cells respectively. Compound 3 showed selective cytotoxic activity to EJ cells ( IC50 7.20 μg/ml). Compound 7 showed cytotoxic activity of 8.53μg/ml ( IC50 ) in Hepg2 cells.