一种人工合成Tuftsin类似肽大鼠组织分布和排泄研究

摘要

目的 研究Tuftsin类似肽(T肽)单次皮下注射后在大鼠体内的组织分布特点和排泄情况.方法 Wistar大鼠,单次皮下注射剂量21.6 mg ·kg-1T肽.采用竞争ELISA方法检测各时间点的组织器官、尿、粪和胆汁中的药物含量.结果 组织分布试验结果表明,药物主要分布在大鼠的血清、肾、脾和小肠组织中,除小肠达峰时间为给药后2h之外,其它药物含量均于给药后15 min达最高；排泄实验结果表明,0～144 h内尿和粪中排泄的原型药物分别占给药总量的2.21％和0.03％；0～96 h内大鼠胆汁中排泄的原型药物为给药总量的0.02％.结论 T肽给药后在Wistar大鼠体内广泛分布,在血清、肾和脾组织中含量较高,在粪和胆汁中检测出T肽的含量很少.%Objective To investigate the biodistribution and excretion of a Synthetic Tuftsin-Analog(T peptide) single subcutaneously administrated in rats.Methods Thirty rats were subcutaneously administered T peptide at a dose of 21.6 mg ·kg-1.The T peptide levels in normal organs,urine,excrement and bile were determined by competitive ELISA method,respectively.Results T peptide was widely distributed in the tissues of rats.The concentration of T peptide in the serum,kidney,spleen and small intestine was high in rats,and reached the maximum at 15 min except for the small intestine at 2 h.The excretion of T peptide in the urine,excrement and bile amounted to 2.21％,0.03％ and 0.02％ of the dose,respectively.Conclusion T peptide is widely distributed in the tissues,especially in the serum,kidney and spleen.A small amount of T peptide is detected in the excrement and bile