Effects of Nivalenol and Selenium on the Metabolism of Aggrecan in the Cultured Human Fetal Chondrocytes

摘要

Objective To investigate the effects of nivalenol(NIV) and selenium(Se) on the metabolism of aggrecan in the cultured chondrocytes,and to explore the mechanism involved in cartilage aggrecan catabolism in the process of Kashin-Beck Disease(KBD).Method Aggrecan mRNA expression was studied by RT-PCR.The concentration of GlcUA in culture medium was determined by diphenylenimine-sulfuric acid method.Result NIV significantly decrease aggrecan mRNA expression.That Se can partially antagonize the effect of NIV on aggrecan mRNA expression.The content of GlcUA in medium with NIV was high er than that in other groups.Conclusion NIV could inhibit chondrocyte synthesis aggrecan,promote the loss of aggrecan from cartilage.All the effects result in the metabolic disorder of the cartilage aggrecan,which eventually leads to irreversible mechani cal destruction of the cartilage.It suggested that Se can partially alleviate the effects of NIV on chondrocytes cultured in vitro.