目的 建立反相高效液相色谱法测定氧氟沙星栓中氧氟沙星的含量.方法 采用C18色谱柱(250 mm×4.6 mm,5 μm),流动相为乙腈-0.05 mol·L-1枸橼酸溶液(三乙胺调节pH4.0)(15∶85);检测波长为293 nm.结果 氧氟沙星质量浓度在20.02～400.3 μg·mL-1范围内呈良好线性关系(r=0.999 7),平均加样回收率为99.3%(n=9),RSD为0.6%.结论该方法简便快速,结果准确,可作为该制剂含量测定的方法.%Objective To establish a high performance liquid chromatography method for determination of ofloxacin in Ofloxacin Suppositories. Methods C18 chromatographic column was used,and the mobile phase was the solution of 0. 05 mol · L-1 citric acid(pH was adjusted to 4. 0 with triethylamine)-acetonitrile (85 : 15) ,and the detection wavelength was 293 nm. Results The linear range of ofloxacin was 20. 02-400. 3 μg ? mL-1 ( r =0. 999 7). The mean recovery was 99. 3%( n =9) with RSD 0. 6 %. Conclusion The method is simple,quick,accurate,and can be used for the quality control of Ofloxacin Suppositories.
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