高良姜素对H2 O2诱导A375细胞氧化损伤的保护作用

摘要

目的：探讨高良姜素对人A375黑素瘤细胞氧化损伤的保护作用。方法以700μmol · L －1的 H2 O2处理人A375黑素瘤细胞，建立氧化损伤模型，并以1，5和10μg · mL －1的高良姜素拮抗其作用。用倒置显微镜观察细胞形态；采用MTT 比色法检测细胞存活率；并检测各组细胞上清液中丙二醛（MDA）、总抗氧化能力（T‐AOC）、过氧化氢酶（CAT）、一氧化氮合酶（NOS）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH‐PX）。结果高良姜素能明显改善 H2 O2损伤后的 A375细胞形态，显著提高人A375黑素瘤细胞的存活率。与模型组相比，高良姜素给药后均能提高 A375细胞 T‐AOC、CAT、SOD和 GSH‐Px 活力（P＜0．05），而MDA和NOS明显降低（P＜0．05）。结论高良姜素对H2 O2诱导的A375细胞氧化损伤具有保护作用。%Objective To investigate the protective effect of galangin on oxidative damage of A 375 induced by H2O2 .Methods A375 oxidative damage cellular model was established by 700 μmol · L -1 H2 O2 treatment .And the damage effect was antagonized by different concentration(1 ,5 and 10 μg · mL -1 ) galangin .The cell morphology was observed by inverted microscope ,the cell sur‐vival was determinated by MTT ,and the changes of MDA ,T‐AOC ,CAT ,NOS ,SOD and GSH‐Px were measured .Results The galangin could significantly improve the cell morphology and the cell survival .Compared with the model group ,galangin could significantly increase the activity of T‐AOC ,CAT ,SOD and GSH‐Px (P<0 .05) ,and the production of MDA and NOS was in‐hibited obviously (P< 0 .05) .Conclusion The galangin has significant protective effect on the A375 oxidative damage cellular model induced by H2 O2 .