Response surface methodology (RSM) was used to optimize ultrasonic-assisted extraction for total polyphenols from Agrimonia pilosa Ledeb. Based on single-factor experiment. The main factors extraction temperature,liquid-material ratio and ethanol concentration were determined. The relationship between influencing factors and response values was established. The optimum extraction conditions were as follows;the extracting solvent was 71% ethanol,solid-liquid ratio was 1:24,extracting temperature was 60 ℃, and extracting time was 20 min. Under these conditions,the maximal yield of total polyphenols reached 3.56%, which was well matched with the predicted content.%在单因素实验的基础上,采用响应面分析法对影响超声辅助提取仙鹤草总多酚得率的主要因素(超声温度、料液比和乙醇浓度)进行优化,建立了影响因素与总多酚之间的函数关系.获得最佳工艺条件为:71％乙醇,料液比1:24,超声提取温度60℃,提取时间20 min.在此最佳条件下,总多酚得率为3.56％,试验结果与模型预测值相符.
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