响应面法优化细果角茴香中总黄酮的超声提取条件

摘要

Response surface methodology(RSM) is applied to optimize ultrasonic-assisted extraction process of total flavonoids from Hypecoum leptocarpum Hook.f.et Thoms.Based on single experiments,the concentration of ethanol,ratio of liquid to solid,and extraction time are selected for Box-Behnken central composite designing.RSM is employed to study the effect of these factors on the yield of total flavonoids.The results indicates that the optimal extraction conditions are 33∶1 mL/g as the ratio of liquid to solid,58％ of aqueous ethanol,with 34.5 min extraction.Under the optimized conditions,the extraction yield of total flavonoids is up to 1.525％.%采用响应面法优化超声波辅助提取细果角茴香中总黄酮的工艺研究.在单因素试验的基础上,选择超声时间、乙醇浓度、料液比为自变量,以总黄酮的提取率为响应值,进行Box-Behnken中心组合实验设计,采用响应面法(RSM)评估了这些因素对总黄酮提取率的影响.结果表明:超声波法辅助提取细果角茴香中总黄酮的最佳工艺条件为液料比33 mL/g,乙醇浓度为58％,提取时间为34.5 min.在此条件下,总黄酮的提取率达1.525％.