细果角茴香中总黄酮的提取及含量测定

摘要

利用热回流法和超声波法提取细果角茴香中的总黄酮，结合NaNO2-Al（NO3）3-NaOH体系分光光度法测定细果角茴香中的总黄酮含量，并对细果角茴香中总黄酮的提取方法进行优化。结果表明：利用超声波法提取细果角茴香中的总黄酮效果较好；且在甲醇浓度为70％、料液比为1：20、超声波提取2次、提取时间分别为2．0h和1．5h的条件下提取效果最好，测得总黄酮的含量为15．44mg／g。NaNO2-Al（NO3）3-NaOH体系分光光度法操作简捷，测定结果准确可靠。%Total flavonoids in H. leptocarpum was extracted by hot reflux method and ultrasonic extraction method, and its content was determined by NaNO2-Al （NO3 ）3-NaOH spectrometry. The results showed that ultrasonic method was more suitable than hot reflux method, and the optimum extraction condition was 70% methanol solution, solid-liquid ratio 1：20 （W/V）, ultrasonic treatment twice with the time of 2. 0 h and 1.5 h. Total flavonoids content in H. leptocarpum was about 15.44 mg/g. NaNO2-Al （NO3 ） 3-NaOH spectrometry method was easy to operate and the results were accurate.