Plackett-Burman和Box-Behnken试验设计优化超声波-酶法提取青钱柳多糖工艺及结构初探

摘要

To develop the resource of Cyclocarya paliurus polysaccharides (CPP),ultrasonic and enzyme-assisted extraction of CPP was optimized and its structure was preliminarily studied.Firstly,the optimum range of each single factor was identified by single factor experiment.Then,the key factors affecting the yield of CPP were screened with Plackett-Burman design,and the extraction of CPP was optimized with Box-Behnken experiment.Finally,UV,FTIR spectra and gas chromatograms were employed to preliminarily identify the structure of CPP.Results indicated that the key factors were pH,amount of mixed enzyme,enzymatic time and ultrasonic power.In addition,the optimal extraction conditions were pH of 6.11,amount of mixed enzyme of 4.81％,enzymatic time of 1.685 h,ultrasonic power of 96.9 W,solid to liquid ratio of 1∶20,and ultrasonic time of 30 min.Under these conditions,the extraction yield of CPP was 7.18％.CPP was a typical polysaccharide and the monosaccharide composition was consisted of rhamnose,arabinose,xylose,mannose,glucose,galactose,with a molar ratio of 1.00∶ 7.03∶ 2.06∶2.11 ∶ 5.25∶ 12.06.%为了开发利用青钱柳多糖(CPP)资源,本文探究了超声波结合复合酶处理提取CPP的工艺条件并对其结构进行了初步分析.首先通过单因素实验获得各因素的最佳范围,利用Plackett-Burman试验设计筛选影响CPP得率的关键因素,再采用Box-Behnken设计对提取工艺进行优化.最后,采用紫外、红外光谱以及气相色谱对CPP结构进行初步分析.结果表明,关键因素为酶解pH、复合酶添加量、酶解时间和超声功率.最佳工艺参数为酶解pH值6.11,复合酶添加量4.81％,酶解时间1.685 h,超声功率96.9 W,料液比1∶20,超声时间30min,CPP得率为7.18％.CPP具有多糖典型的羟基和醛基结构,且单糖组成为鼠李糖∶阿拉伯糖∶甘露糖∶葡萄糖∶半乳糖=1.00∶7.03∶2.06∶2.11∶5.25∶12.06.