黄芩饮片、标准汤剂、中间体、配方颗粒的HPLC指纹图谱相关性研究

摘要

建立黄芩饮片、标准汤剂、中间体、配方颗粒的HPLC指纹图谱,并对其相关性进行评价.采用HPLC法,色谱柱为Agilent Eclipse XDB C18色谱柱(250 mm×4.6 mm,5μm);甲醇:0.05％磷酸水作为流动相;流速1.0 mL/min;检测波长275 nm;柱温35℃;进样体积10μL.黄芩10批饮片、标准汤剂、中间体及配方颗粒指纹图谱均呈现9个共有特征峰,呈良好的相关性,在9个共有峰中指认出黄芩苷、黄芩素、汉黄芩素3个成分.黄芩饮片、标准汤剂、中间体、配方颗粒的主要化学成分组成基本相同,建立的HPLC指纹图谱方法可用于黄芩配方颗粒的生产全过程的质量控制.%In this study,the HPLC fingerprint method of the pieces,standard decoction,intermediates and dispensing granules of Scutellaria baicalensis was established,and the correlations among them were evaluated.The chromatographic separation was carried out on an Agilent Eclipse XDB C18 column (250 mm ×4.6 mm,5 μm);The mobile phase was eluted with gradient by methanol-0.05 ％ phosphoric acid;The flow rate was 1.0 mL/min.The column temperature was 35 ℃ and the detection wavelength was set at 275 nm.There were 9 common characteristic peaks in all fingerprint spectrum of 10 batches of pieces,standard decoction,intermediates and dispensing granules of S.baicalensis,presenting a good correlation,in the 9 common characteristic peaks,three of them were baicalin,baicalein,wogonin.The main chemical constituents of S.baicalensis pieces,standard decoction,intermediates and dispensing granules composition are basically identical.The established HPLC fingerprint method can be used for the quality control of S.baicalensis dispensing granules.