目的 分析杀伤细胞免疫球蛋白样受体(KIR)基因型和单倍型在强直性脊柱炎患者中的分布特点.方法 采用序列特异性引物聚合酶链反应方法 ,分析105例强直性脊柱炎患者、62例骨关节炎患者和412名健康对照KIR基因型和单倍型.结果 (1)强直性脊柱炎组KIR基因型3DL3-2DL3-2DL1-2DP1-2DL4-3DL1-2DS4-3DL2阳性率(6.67%)明显低于健康对照组和骨关节炎组(20.15%,17.74%;P=0.001,0.037).(2)强直性脊柱炎组KIR基因型3DL3-2DL3-2DL2-2DL1-2DP1-2DL4-3DL1-2DL5-2DS1-2DS2-2DS3-2DS4-2DS5-3DS1-3DL2阳性率(9.52%)及基因型3DL3-2DL3-2DL2-2DL1-2DP1-2DL4-3DL1-2DL5-2DS1-2DS4-3DL2阳性率(5.71%)明显高于健康对照组(2.18%,0.49%;P=0.001,0.001),骨关节炎组未检出这2种基因型.(3)三组人群KIR单倍型差异无统计学意义.结论 KIR基因型分布异常,可能是强直性脊柱炎的免疫遗传因素,其分子机制有待进一步研究.%Objective To investigate the association of killer cell immunoglobulin-like receptors (KIR) genotype and haplotype with ankylosing spondylitis (AS). Methods Peripheral blood samples were collected from 105 AS patients, 62 patients of osteoarthritis (OA), and 412 randomly selected healthy controls. Polymerase chain reaction with sequence-specific primers (PCR-SSP) was used to detect the KIR genotype and haplotype. Results The genotype frequency of 3DL3-2DL3-2DL1-2DP1-2DLA-3DL1-2DS4-3DL2 (6.67%)was significantly lower in the AS patients than in the control subjects (20.15% ) and OA patients( 17.74%, P = 0. 001,0. 037 respectively). The genotype frequency of 3DL3-2DL3-2DL2-2DL1-2DP1-2DL4-3DL1-2DL5-2DS1-2DS2-2DS3-2DS4-2DS5-3DS1-3DL2 and 3DL3-2DL3-2DL2-2DL1-2DP1-2DL4-3DL1-2DL5-2DS1-2DS4-3DL2 of the AS patients (9.52% ,5.71% )was significantly higher than that of the controls (2.18 % ,0.49 % ; P = 0.001,0.001 ), and these two genotypes were not detected in the OA patients. There were not significant differences in the haplotypes A and B among the AS patients, OA patients, and healthy controls. Conclusion KIR genotypes may be associated with the susceptibility to AS.
展开▼
