响应面法优化干蟾皮的提取工艺

摘要

目的:优选干蟾皮的提取工艺条件.方法:采用回流提取法提取干蟾皮,以华蟾酥毒基和酯蟾毒配基的总量为考察指标,运用响应面法的设计,考察乙醇浓度、液料比、提取时间对提取工艺的影响.结果:优化的提取工艺条件为乙醇浓度为88.64％,液料比为12∶1,提取时间为1.49 h,在该优化条件下,华蟾酥毒基和酯蟾毒配基的总量为2.24 mg.结论:该优化工艺简便、预测性良好,可以为干蟾皮的开发提供参考.%Objective:To optimize the extraction process of craft bufo.Methods:The refluxing process was applied,the amount of cinobufagin and resibufogenin was used as response value,box-Behnken response surface method was employed to investigate the influence of ethanol concentration,extraction time and the amount of alcohol added on the extraction process of craft bufo.Results:The optimized extraction technology was:extracted 2 times with 12 times the amount of 88.64％ ethanol;1.49 hours per time.Under the optimized conditions,the total amount of cinobufagin and resibufogenin was 2.24 mg.Conclusion:The optimized extraction process is simple and predictable,can provide a reference basis for production.