粪肠球菌F71的增殖培养基优化研究

摘要

为达到增殖菌体的目的，对粪肠球菌F71进行了培养基优化正交试验。结果表明，最佳培养基的组成为：胰蛋白胨10 g/L，酵母粉10 g/L，葡萄糖 10 g/L，无水氯化钙0.04 g/L，七水合硫酸镁0.019 2 g/L，磷酸氢二钾0.04 g/L，磷酸二氢钾0.04 g/L，磷酸氢钠0.4 g/L，氯化钠0.08 g/L，土豆汁50 g/L，碳酸钙1 g/L，乙酸钠10 g/L，碳酸铵2 g/L，乙酸铵1 g/L，半胱氨酸盐酸盐0.5 g/L，pH值为9，最适培养温度为35 ℃。用此培养基进行增殖，F71的最高活菌数达到2.78×108 cfu/mL，比基础培养基提高了54.6%。%In order to realize the multiplication of isolates,the optimization experiment of medium for Enterococcusfaecalis F71 was carried out by orthogonal design. The optimization of cultured medium of F71 was as follows：peptone 10 g/L,yeast extract l0 g/L,glucose 10 g/L, anhydrous calcium chloride 0.04 g/L,bitter salt 0.019 2 g/L,dipotassium hydrogen phosphate 0.04 g/L ,monopotassium phosphate 0.04 g/L,monosodium phosphate 0.4 g/L,sodium chloride 0.08 g/L,juice of potato 50 g/L,calcium carbonate 1 g/L,sodium acetate 10 g/L,ammonium carbonate 2 g/L,ammonium acetate 1 g/L, cysteine hydrochloride 0.5 g/L. The optimal initial pH value of the medium was 9, F71 got to 2.78× 10^8 cfu/mL in this culture for 16 hours at 35 ℃ ,and increased by 54.6% compared to the basic culture medium.