目的：探讨自噬相关因子LC3、Bnip-3及凋亡相关因子Bcl-2、Bax在实验性糖尿病大鼠脑组织损伤中的作用。方法30只SD大鼠随机分为糖尿病组和对照组。糖尿病组一次性腹腔注射1％链脲佐菌素（60 mg／kg体质量），对照组一次性腹腔注射柠檬酸缓冲液，饲养12周后活杀大鼠，留取脑组织标本。经HE染色观察其病理学改变，采用免疫组化S-P法检测大鼠脑组织中LC3、Bnip-3、Bcl-2及Bax的表达。结果与对照组大鼠相比，糖尿病大鼠脑组织病理学显示细胞排列紊乱，分布不均，胞体缩小，胞质淡红色，正常形态神经细胞数减少；LC3、Bnip-3及Bax阳性数较对照组明显升高，差异有统计学意义（ P＜0．05）；Bcl-2阳性数较对照组明显降低，差异有统计学意义（P＜0．001）；LC3与Bnip-3呈弱正相关（P＜0．05），与Bcl-2及Bax不相关；Bnip-3与Bax呈正弱相关（P＜0．05），与Bcl-2无相关；Bcl-2与Bax无相关。结论糖尿病大鼠脑组织中LC3、Bnip-3及Bax存在过度表达，Bcl-2呈弱表达，表明自噬因子与凋亡因子参与了糖尿病大鼠脑组织损伤的过程，可能是脑组织损伤的机制之一。%Objective To evaluate the effect of autophagy-related factors LC3 and Bnip-3 and apoptosis related factors Bcl-2 and Bax on brain damage in experimental diabetic rats .Methods Thirty SD rats were randomly divided into diabetic group and control group .The diabetic group was injected with 1% streptozotocin ( 60 mg/kg body mass ) and the control group with citrate buffer .The rats were sacrificed after 12 weeks feeding and brain tissues were obtained .Pathological chan-ges were observed and the expression of LC 3, Bnip-3, Bcl-2 and Bax in brain tissues of the rats was detected by immuno-histochemical SP method .Results Compared with the control group ,the diabetic rat brain pathology showed that the cell arrangment was more disorderly and distributed more unevenly , the cell body was smaller , cytoplasm was lighter red , and the number of nerve cells of normal morphology was smaller .The positive number of LC3, Bnip-3 and Bax in brain tissues of diabetic rats was significantly larger than in the control group ,and the difference was statistically significant (P<0.05). However ,the positive number of Bcl-2 was significantly smaller than in the control group ,and the difference was statistically significant (P<0.001).In diabetic rats, LC3 and Bnip-3 showed a weak positive correlation(P<0.05), Bcl-2 and Bax were irrelevant, Bnip-3 and Bax were positively weakly-correlated(P<0.05),Bcl-2 was not correlated;and Bcl-2 and Bax were irrelevant.Conclusion LC3,Bnip-3 and Bax in the brain tissue of diabetic rats are overexpressed , while Bcl-2 shows weak expression , indicating that autophagy factors and apoptotic factors are involved in the process of brain injury in diabet -ic rats, which may be one of the mechanisms of brain tissue damage .
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