METHOD FOR INDUCING SYNERGISTIC EXPRESSION OF SPECIFIC GENE USING DEMETHYLASE AND TRANSCRIPTION-ASSOCIATED FACTOR OR CHROMATIN-ASSOCIATED FACTOR

摘要

As therapy using the overexpression of endogenous genes becomes more and more important, there has been required, as a novel gene therapy tool, a small-sized system by which a potent gene can be activated and cloned into a viral vector. The present invention provides a system by which an endogenous gene can be overexpressed. More specifically, provided is a modified SunTag system by which a transcription-associated factor or a chromatin-associated factor and a demethylase are both introduced into a target gene with the use of dCas9-SunTag. According to this system, gene expression can be significantly activated compared to the introduction of the transcription-associated factor or the chromatin-associated factor alone or the demethylase alone. The introduction of the combination of a transcription-associated factor or a chromatin-associated factor with a demethylase using dCas9-SunTag can promote the expression of genes over a wide range, which makes this system applicable to broader targets.