流式细胞术检测41例维族尔族骨髓增生异常综合征患者有核红细胞免疫表型的临床意义

摘要

Objective:To research the characteristics of immunophenotype of nucleated erythrocytes in 41 Uyghur patients with Myelodysplastic syndrome (MDS) and analyze their clinic significance. Methods: The expressions of antigens of nucleated erythro-cyte population in 41 patients with MDS were detected by multiparametric flow cytometry. Results: Comparing with the non- MDS group, the proportion of nucleated erythrocyte population was significantly higher in the MDS group ( =0.029) . The proportion of nucleated erythrocyte population was no significant differences in RCMD, RAEB- I and RAEB- II ( ﹥0.05) . The expressions of GPA+CD71+, CD71, CD105 and GPA on nucleated erythrocytes were significantly increased than non- MDS group ( =0.026, =0.020, =0.033, and =0.024, respectively) , while the expressions of GPA+CD105+ and CD71+CD105+ were no significantly ( =0.050, =0.938, respectively) . Expressions of all other antigens were no significantly differences among the subtypes of MDS ( ﹥0.05) . Conclusion: Multiparametric flow cytometry ( FCM) can reveal the characteristics of immunophenotypic abnormalities of nucleated erythrocytes in Uyghur patients with MDS and provide more objective information for the diagnosis and prognosis.%目的：探讨维吾尔族骨髓增生异常综合征（myelodysplastic syndrome，MDS）患者有核红细胞免疫表型特点及其在诊断和预后中的临床意义。方法：应用多参数流式细胞术（flow cytometry，FCM），对41例MDS 患者和30例非 MDS （non- MDS）患者有核红细胞免疫标志的表达率进行检测。结果：MDS 与non- MDS相比，有核红细胞群比例显著升高（=0.029），在MDS亚型间无显著差异（＞0.05）；有核红细胞群中GPA+CD71+、CD71、CD105和GPA表达均显著升高（值分别为：0.026、0.020、0.033和0.024）， GPA+CD105+和CD71+CD105+表达均无显著差异（值分别为：0.050和0.938）；其余抗原亚型间比较均无显著差异（＞0.05）。结论：多参数FCM能够检测到维吾尔族MDS患者有核红细胞异常免疫表型特点，为临床上对MDS患者诊断和预后评价提供更多有价值的客观信息。