Objective To explore the effect of recombinant human granulocyte macrophage colony stimulating factor (rhGM CSF) hydrogel on deep partial thickness burn and the mechanism of action on promoting wound healing by establishing the model of deep partial burn in rats, coated with rhGM CSF hydrogel and bandaged with iodophor gauze. Methods 40 rats were randomly divided into experimental and control groups. The experimental and control groups treated respectively with rhGM CSF hydrogel and iodine solution of 0. 5 ‰ once a day for 13 days. Select 5 rats from each group randomly to execute on 4th, 7th, 10th and 13th day after administration respectively. Then evaluate the calculations of wound healing rate (WHR) , the count of fibroblasts (FB) , the area of the collagen fibers and wound microvascu-lar density (MVD). Results The calculations of wound healing rate (WHR) :the difference of WHR were not statistically significant on 4th day(P>0. 05),the WHR in the experimental group were obviously higher than that in the control group on 7th,10th day 13th day(P<0. OS). The count of fibroblasts (FB):the FB in the experimental group was obviously higher than that in the control group on 4th,7th and 10th day after treatment (P<0. 05). Nevertheless, which in the control group was obviously higher than that in the experimental group on 13th day after treatment (P<0. 05). The area of the collagen fibers: The area of the collagen fibers in the experimental group was obviously higher than that in the control group on 4th,7th, 10th and 13th day after treatment (P<0. 05). 4. Wound microvascular density (MVD): The number of microvessels in the experimental group were obviously higher than that in the control group on 4th,7th, 10th and 13th day after treatment (P<0. 05). Conclusion rhGM CSF hydrogel by promoting microvascular growth, promoting fibroblast formation and wound collagen formation to increase wound healing and improve the repaired quality. Compared to conventional treatment methods, rhGM CSF hydrogel can significantly accelerate wound healing on deep partial burn.%目的 探讨重组人粒细胞-巨噬细胞集落刺激因子凝胶对深Ⅱ度烫伤创面的影响及促进创面愈合的作用机制.方法 40只大鼠采用相同的方法在背部制成深Ⅱ度烫伤,随机分成实验组和对照组,实验组为rhGM-CSF凝胶治疗组,对照组为0.5‰碘溶液治疗组,各组每天给药1次,连用13天.伤后4、7、10、13天各组随机选取5只大鼠处死,计算创面愈合率、成纤维细胞含量、胶原含量和微血管密度计数.结果 伤后4天,实验组与对照组创面愈合率比较无明显差异(P＞0.05)；伤后7、10、13天,实验组均优于对照组(P＜0.05).伤后4、7、10天,实验组成纤维细胞含量均高于对照组(P＜0.05),但伤后13天,实验组低于对照组(P＜0.05).伤后4、7、10、13天,实验组胶原含量均高于对照组(P＜0.05).伤后4、7、10、13天,实验组微血管密度计数均高于对照组(P＜0.05).结论 外用重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)通过促进微血管生长、促进成纤维细胞的形成及创面胶原的形成来增加创面愈合速度及改善修复质量,与传统的外用碘伏纱布包扎换药治疗相比能明显缩短治疗过程.
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