Objective: To evaluate the effect of rhGM-CSF on scalding wound healing and neovascularization. Methods: Deep Ⅱ degree bum wound was made in72 SD rate on the back with diameter of3 cm. Rate were randomly divided into experinent group( n = 36, local wound was treated with rhGM-CSF gel) and control group(n =36, bcal wound were treated with gel matrix without rhGM-CSF). The process of wound healing was observed, and the percentages of wound closure were calculated on the day, the 1st,2 nd,3 rd, 7 th,10 th,14 th, and 21 st day after scald The expression of CD31, the surfacemaker of neovascularendothelial cells was detetcted with in the wound sites by immunoh istochamical staining, and the microvessel density was calculated. Results: The percentages of wound closure in experinent group were significantly higher than that in control group from the 7 th day to the21 st day. Immunohistochanical detection revealed that the expression of CD31 and the microvessel densily in experinent group were significantly higher than those in control group from7 th day to21 stday(P <0.01). Conclusions: The treatment of rhGM-CSF may promote scalding wound healing in rate The mechanisn may be associated with upregulation of CD31 expression and acceleration of neovascularization.%目的:研究重组人粒细胞巨噬细胞集落刺激因子(recombinant human granulocyte-macrophage colony-stimulating factor,rhGM-CSF)对烫伤创面组织愈合及新生血管化的影响.方法:72只SD大鼠于背部制备直径3 cm的深Ⅱ度烫伤创面.根据创面不同处理方式将大鼠随机分为实验组(创面局部应用rhGM-CSF凝胶剂)和对照组(创面局部应用不含rhGM-CSF的凝胶基质),每组36只.分别于创面形成后当日和第1、3、7、10、14、21天观察创面并计算创面愈合率;免疫组织化学染色观察创面组织中新生血管内皮细胞表面标志物CD31表达,计算微血管密度.结果:自创面形成后第7天起,实验组创面愈合率明显高于对照组(P<0.01),实验组创面组织中CD31表达及微血管密度也均明显高于对照组(P<0.01).结论:外用rhGM-CSF具有促进烫伤创面愈合作用,其机制可能与上调创面组织中CD31表达、使新生血管再生加速有关.
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