多发性硬化患者外周血调节性T细胞功能受损

摘要

Objective:To detect regulatory T cells and Foxp3 expression in peripheral blood of patients with multiple sclerosis (MS).Methods:A total of 58 MS patients and 24 healthy people were included in this study,the number of CD4 + CD25 + regulatory T cells in peripheral blood were detected by flow cytometry,Foxp3 expression were analyzed by real-time fluorescent quantitative RT-PCR.Results:There was no difference of the number of Treg cell between MS patients and healthy people,but the expression of Foxp3 were significantly decreased in MS patients(P ＜ 0.01).Conclusion:The function of Treg cells were severely impaired in MS patients although its number had not changed compared with healthy people,which could contribute to the onset of this autoimmune disease.%目的:探讨多发性硬化患者外周血调节性T细胞(Treg)的变化及其转录因子Foxp3的表达.方法:收集58例多发性硬化(MS)患者及24例正常人外周血,通过流式细胞术检测CD4+ CD25+T细胞,RT-PCR检测Foxp3的表达.结果:MS患者外周血中Treg细胞数量较正常人无差异(P＞0.05),而Foxp3表达明显减低(P＜0.05).结论:MS患者外周血中Treg细胞数量较正常人无明显变化,但其功能明显受损,是导致自身免疫性疾病发生的原因之一.