空泡型质子泵与转化生长因子-β1在胃癌中表达及意义

摘要

目的：探讨空泡型质子泵（V-ATPases）与转化生长因子-β1（TGF-β1）在胃癌组织中的表达，并探讨其临床意义。方法：运用免疫组化方法检测胃癌组织及癌旁组织中V-ATPases与TGF-β1的表达水平，并分析两者相关性及与胃癌临床病理参数之间的关系。结果：免疫组化结果显示，（1）V-ATPases表达胃癌组织45例中阳性34例（75.5%），癌旁组织阳性13例（28.8%），两者比较差异有统计学意义（P<0.05）。（2）TGF-β1表达胃癌组织阳性35例（77.8%），癌旁组织阳性15例（33.3%），两者比较差异有统计学意义（P<0.05）。（3）相关性分析结果：V-ATPases的表达与TGF-β1的表达呈正相关（P<0.05）；V-ATPases与TGF-β1的表达与病理分级、淋巴结转移、TNM分期、脉管浸润呈正相关（P<0.05），与临床病理特征如性别、年龄、肿瘤大小无相关性。结论：在胃癌组织中高表达的V-ATPases与TGF-β1可能胃癌发生浸润转移的重要生物学标志，检测V-ATPases与TGF-β1对判断胃癌浸润转移具有重要意义。%Objective: To investigate the expression of V-ATPases and TGF-β1 in gastric cancer and their clinical significance. Methods: Immunohistochemistry and western blot were used to detect the expression of V-ATPases and TGF-β1 in gastric cancer specimens, respectively. The correlations among the V-ATPases, TGF-β1 protein, and clinico-pathological parameters of gastric cancer were analyzed. Results: In the assay of immunohistochemistry showed that the positive expression rates of V-ATPases were 75.5%and 28.8%in gastric cancer and the adjacent tissue, respectively. The positive expression rates of TGF-β1 were 77.8 % and 33.3 % in gastric cancer and the adjacent tissue, respectively. Sig-nificant differences (P<0.01) were found between the two aforementioned groups. The correlation analysis indicated that the expression of V-ATPases had a close positive correlation with that of TGF-β1 in gastric cancer (P<0.01). The up-regula-tion of V-ATPases expression was associated with lymph node metastasis, TNM, vascular invasion and differentiation (P<0.01). The expression of the TGF-β1 expression was associated with an advanced clinical stage, infiltrating depth, lymph node metastasis and vascular invasion in the cancer (P<0.01). Conclusion:The high expression of V-ATPases and t TGF-β1 protein may be important biological markers for malignant transformation in the invasion and metastasis of gastric can-cer. The detection of V-ATPases and TGF-β1 expression in gastric cancer is significantly important for improving the pre-diction of invasion and metastasis.