研究植物乳杆菌对肠道细菌易位的抑制作用

摘要

目的 研究植物乳杆菌对肠道细菌易位的抑制作用.方法 将SD大鼠随机分为4组,即假手术对照组,乳杆菌L2灌胃组,缺血再灌注(Ischemia/Reperfusion,I/R)组,乳杆菌预处理+缺血再灌注组.观察各组动物肠黏膜形态病理学变化、肠道菌群变化、细菌易位、血浆细胞因子的水平.结果 与假手术对照组相比,缺血再灌注组大鼠肠黏膜有明显病理损伤改变,肠黏膜出现坏死、脱落.肠道内厌氧菌数量显著下降,肠杆菌数量也有所下降.在肝、脾、肾及肠系膜淋巴结中有细菌存在,其易位率达87.5％ (P＜0.01).乳杆菌灌胃组大鼠各项指标无显著变化,乳杆菌预处理+I/R组其变化程度较之缺血再灌注组显著减弱.结论 植物乳杆菌L2能够抑制肠道缺血再灌注损伤引起的细菌易位,减弱其对肠黏膜屏障的损伤.%Objective To investigate the ability of Lactobacillus plantarum L2 to prevent intestinal ischemia and reperfusion induced bacterial translocation.Methods 32 SD rats were randomly divided into four groups:sham,Lactobacillus intragastric administration,ischemia and reperfusion (I/R) model,Lactobacillus pre-treatment and I/R model.The bacterial translocation,changes of gut microflora,levels of proinflammatory cytokines in plasma and intestinal morphology were observed.Results The increase in bacterial translocation was significant after 4 h I/R treatment (P ＜ 0.01) as compared with sham-operated group.There were obvious pathological changes of mucosal morphology in I/R rats and the number of anaerobic bacteria such as Bifidobacteria,Lactobacillus and Bacteroides was significantly reduced.The level of TNF-α in plasma was increased after I/R injury.Lactobacillus plantarum treatment alone had no effect on intestinal barrier function.However,pretreatment of rats with Lactobacillus plantarum L2 significantly abrogated I/R induced intestinal mucosal injury and prevented translocation of bacteria to mesenteric lymph nodes or to remote organs.Conclusions Lactobacillus plantarum L2 has the ability to prevent bacterial translocation and attenuate I/R induced intestinal injury.