甲状腺乳头状癌miR-34b基因表达及其启动子区甲基化的临床意义研究

摘要

目的：探讨miR-34b基因表达及其启动子区甲基化（MSP）在甲状腺乳头状癌（PTC）中的临床意义。方法：收集2008年10月-2013年1月本院治疗的42例甲状腺乳头状癌患者癌组织标本（实验组）和42例甲状腺腺瘤患者瘤旁组织标本（对照组），检测两组miR-34bmRNA表达（实时定量PCR法）及miR-34b基因启动子区甲基化（甲基化特异性PCR法），并对结果进行分析。结果：实验组miR-34b mRNA相对平均表达量（0.84±0.04），miR-34b基因启动子甲基化阳性率71.4%，与对照组比较，差异有统计学意义（P<0.05）；实验组中不同性别、年龄、肿瘤直径、分期和包膜浸润之间，甲基化阳性率比较差异无统计学意义（P>0.05）；而存在淋巴结转移甲基化率（88.5%）显著高于不存在淋巴结转移（43.8%），差异有统计学意义（P<0.05）。结论：miR-34b基因表达及其启动子区甲基化与PTC发生发展具有密切关系，建议临床深入研究。%Objective:To discuss the clinical significance of miR-34b gene expression and its promoter region methylation(MSP)in papillary thyroid carcinoma(PTC). Method:From October 2008 to January 2013,carcinoma tissue specimens(the control group)in 42 patients with papillary thyroid carcinoma and pericarcinomatous tissue specimens (the experimental group)in 42 patients with thyroid adenoma were collected,then miR-34b mRNA expression(real time quantitative PCR method)and miR-34b gene promoter region methylation(methylation-specific PCR method)of two groups were detected and analyzed.Result:In experimental group,the average expression level of miR-34b mRNA was(0.84±0.04), the positive rate of miR-34b gene promoter methylation was 71.4%. Compared with the control group,there were statistically significant difference(P<0.05). In the experimental group,the methylation rate were no significant difference between different gender,age,tumor diameter,stage,and capsular invasion(P>0.05). However,the rates of lymph node metastasis methylation was(88.5%),it was significantly higher than that of no lymph node metastasis(43.8%),there was a statistically significant difference(P<0.05).Conclusion:There is a close relationship between miR-34b gene expression and promoter region methylation development,a deep clinical study is recommended.