金华火腿粗肽液的体外抗氧化活性

摘要

在金华火腿长期加工过程中,蛋白质在内源酶作用下产生了大量不同长短的肽段.提取金华火腿粗肽液,通过测定不同质量浓度粗肽液清除自由基、螯合金属离子、抑制脂质过氧化和蛋白质氧化的能力,并与相应质量浓度的丁基羟基甲苯(BHT)和谷胱甘肽(GSH)作比较,衡量金华火腿粗肽液的抗氧化能力.结果表明:随着质量浓度的增加,金华火腿粗肽液的抗氧化能力显著增加.在质量浓度为1.5mg/mL时,金华火腿粗肽液清除羟基自由基能力达到90％；质量浓度为5mg/mL时,金华火腿粗肽液清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基能力与BHT和GSH相当,高达95％;金华火腿粗肽液螯合金属离子的能力显著高于BHT和GSH;当质量浓度为4mg/mL时,金华火腿粗肽液具有和BHT和GSH相当的抑制脂质过氧化能力,并能一定程度抑制蛋白质氧化.结论:金华火腿粗肽液的抗氧化能力与BHT与GSH相当.%Endogenous proteolysis generates a large number of peptides of different chain length during the long ripening period of Jinhua ham,some of which have potent antioxidant activity.In the present study,crude peptides were extracted from Jinhua ham and evaluated for antioxidant activity by radical scavenging,metal ion chelating,anti-lipid peroxidation,anti-protein oxidation assays with BHT and GSH as controls.Results showed that the crude peptide extract from Jinhua ham had concentration dependent antioxidant activity; the hydroxyl radical scavenging capacity was 90％ at 1.5 mg/mL,and the DPPH radical capacity (as high as 95％) at 5 mg/mL was comparable to that of BHT and GSH.In addition,the crude peptides had much higher metal ion chelating activity than both antioxidants,at 4 mg/mL had similar anti-lipid peroxidation activity to BHT and GSH,and presented an inhibitory effect on protein oxidation.In conclusion,the crude peptides extracted from Jinhua ham have similar antioxidant activity to BHT and GSH.