高效液相色谱法同时测定糯米酒中17种氨基酸

摘要

RP-HPLC has been developed for simultaneous determination of 17 kinds of amino acids content in yellow rice wine. OPA reagent was used for post-column derivatization and RP-HPLC gradient elution was operated before the determination. The determination results suggested that the linear correlation coefficient r was within 0.9990-0,9998, the average recovery was 94.5 %-98.7 %, and precision RSD was 0.51%- 1.90 %. Such method was highly specific and easy to operate with high sensitivity. It could be used for the determination of amino acids in yellow rice wine.%建立反相高效液相色谱法（RP—HPLC）用以测定糯米酒中的17种氨基酸。采用OPA试剂柱后衍生，RP—HPLC经梯度洗脱后检测糯米酒中17种氨基酸的含量。结果表明，该方法测定17种氨基酸的线性相关：象数R在0．9990-0．9998，平均回收率为94．5％～98．7％，精密度RSD为0．51％～1．90％。该方法专属性强、操作方便、灵敏度高，可用于糯米酒和花雕酒等黄酒中17种氨基酸含量的检测。