建立了一种快速简便定量白酒中四甲基吡嗪(TMP)的高效液相色谱-质谱(HPLC-MS/MS)检测方法,并对宣酒小窖芝麻香型白酒蒸馏过程中不同层次馏分酒TMP含量的变化规律进行了分析研究.实验结果表明,该方法相关系数R2为0.9983,检测限为0.25μg/L,定量限为0.85μg/L,在不同样品中的回收率为92%~104%,相对标准偏差为0.51%~3.26%,该方法简单、快速、灵敏、准确度高,满足白酒中TMP的检测要求.宣酒小窖芝麻香型白酒不同层次糟醅馏分酒中TMP变化规律明显,丢糟中含量最高,中糟中最低;各馏分酒中TMP含量随蒸馏时间的延长呈不断上升的趋势.快速分析芝麻香型白酒蒸馏过程中TMP含量的变化规律,为量质摘酒、提高酒质提供了依据.%A rapid detection method of tetramethylpyrazine (TMP) in Baijiu by HPLC-MS/MS had been developed, which was used to detect TMP content in different distillate during the distillation of Zhimaxiang Baijiu. The experimental results showed that such method had good lin-ear relationships (R2=0.9983 and detection limit=0.25 μg/L) with its quantitative limit as 0.85 μg/L. The relative standard deviation was be-tween 0.51%-3.26%and the recovery was within 92%-104 %. The method was simple, rapid, sensitive and precise. It was suitable for the detection of TMP in Baijiu. There was significant difference in TMP content in different distillate during the distillation of Zhimaxiang Baijiu (TMP content the highest in waste fermented grains and the lowest in medium fermented grains). Besides, TMP content increased with distilla-tion time. The analytic results provided theoretical evidence for improving liquor quality and liquor-receiving according to quality.
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