红麻线粒体中细胞质雄性不育候选基因cox3的克隆与分析

摘要

Objective: To compare the difference of the mitochondrial DNA(mtDNA) of kenaf between in cytoplasmic male sterility (CMS) and its maintainer lines and to clone cox3 gene in CMS. Elucidating the mechanism of CMS of kenaf should be propitious to make good use of its heterosis. Methods: mtDNA were isolated to carry out Southern blot by using cox3 gene as probe. The cox3 gene was amplified with PCR by using a homology methold.Results: mtDNA showed significant different in CMS and maintainer lines. The cox3 gene was cloned successfully in kenaf, and the gene showed no difference in CMS and maintainer lines. The gene was submitted to GenBank with the No. HM535784. The gene was 798 bp and shared over 95.9％ identities with other species. Conclusion:The results would provide a basis for cloning the flank sequence of cox3 gene so as to reveal the mechanism of CMS of kenaf.%目的:比较红麻不育系和保持系线粒体基因组的差异,并克隆红麻细胞质雄性不育候选基因cox3,揭示红麻细胞质雄性不育的分子机理.方法:用Southern印迹方法研究红麻保持系和不育系线粒体基因组的差异;用同源克隆的方法克隆cox3基因.结果:不育系和保持系基因组存在较大差异;在保持系和不育系中克隆了cox3基因,其基因CDS区完全一致,基因长度为798 bp,GenBank序列号为HM535784;cox3基因与其他物种的cox3基因的同源性大于95.9%.结论:cox3基因的组织形式在不育系和保持系中存在差异,研究结果为揭示红麻细胞质雄性不育的机理提供了一定的依据.