兔抗伤寒杆菌免疫血清在免疫学检验实验教学中的应用

摘要

Objective To investigate the preparation method of high titer rabbit anti‐Salmonella typhi immune serum .Methods The rabbits were grouped .Using short‐range immunization ,with the typhoid bacillus “O” and “H”diagnostic liquid as antigen ,after small dose and multiple respective inoculations ,the rabbit immune serum was col‐lected .The serum antibody titer was detected by using the slide agglutination test ,tube agglutination test and indirect agglutination .Results The high titer antibodies in rabbits were produced after immunization and the titers reached positive 1 ∶ 1 280 and 1 ∶ 640 respectively .Conclusion The rabbit immune serum prepared by immunizing rabbits with typhoid bacillus can obtain the high titer immune serum in a short period of time ,which is suitable for the exper‐iment teaching of undergraduate immunology test .%目的：探讨制备高效价兔抗伤寒杆菌免疫血清的方法。方法对家兔分组，采用短程免疫法，用伤寒杆菌“O”和“H”诊断菌液作为抗原，分别小剂量连续多次接种后，收集兔免疫血清，利用玻片凝集实验、试管凝集实验和间接凝集实验，测定血清中抗体的效价。结果家兔在全程免疫后产生了高效价抗体，效价分别达到了阳性，1∶1280、1∶640。结论用伤寒杆菌免疫家兔，制备的免疫血清在短时间内可以获得高效价的抗血清，适用于免疫学检验本科实验教学。