实时荧光定量PCR技术在致病菌检测中的应用

摘要

目的 本文将实时荧光定量PCR (Real-time Q-PCR)法检查致病菌与细菌培养法作比较,探讨对指导临床诊断治疗的意义.方法 采用Real- time Q- PCR法和细菌培养法对100份标本进行6个微生物项目的检测,并比较两种方法的灵敏度、特异性和准确性.结果 Real-time Q-PCR法检测结果与细菌培养法,除完全符合的19例标本之外,另有8例用细菌培养法为阴性的标本,用Real- time Q- PCR法检测为阳性.结论 Real-time Q-PCR法较细菌培养法具有准确性好、灵敏度高、特异性强和检测快速等优点,是快速筛查致病菌的理想检测方法之一.%Objective To establish a rapid screening of pathogenic bacteria by the real- time quantitative PCR to guide the clinical diagnosis and treatment. Methods 100 specimens were tested by bacteria culture method in national standard and the real-time Q-PCR for six microbial items. The sensitivity, specificity and accuracy ofrnthe two methods were compared. Results The accordance rate of the real-time Q-PCR and the bacteria culture in 19 samples was100%. 8 specimens which showed negative with the traditional method were positive by realtime Q- PCR. Conclusion Real- time Q- PCR may be more accuracy, sensitivity, specific, and faster than that the bacteria culture. It may be used as an ideal methods in quick screening the pathogens of patients.