DEVELOPMENT OF GENE SPECIFIC PROBE AND PRIMERS FOR DETECTION OF INDIAN ISOLATE OF MOURILYAN VIRUS IN PENAEUS MONODON SHRIMP BY REAL TIME PCR

摘要

Based on the sequence information obtained for Mourilyan virus G2 protein gene of Indian isolate, a specific real time PCR primers and probe were developed. Mourilyan virus could be detected with increased sensitivity in infected shrimp tissues such as gills, gut, hepatopancreas, muscle and haemolymph. The real time analysis revealed highest Mourilyan virus copy numbers (22.5 x 105) µ-1 cDNA in gill tissue of shrimp, indicating gill tissues to be the appropriate sample for testing presence of MoV by real time PCR.