MEASURING OF TT VIRUS GENE BY REAL TIME DETECTION PCR METHOD AND PRIMER AND PROBE USED THEREFOR

摘要

PROBLEM TO BE SOLVED: To obtain the subject new primer comprising an oligonucleotide having a sequence of continuous specific base numbers in a specific base sequence and useful for measurement of a new hepatitis virus (TTV) gene, or the like, by real time detecting PCR method.;SOLUTION: A new forward side primer comprising an oligonucleotide having a base sequence (in which T may be U) comprising a continuous 15-39 bases in a base sequence represented by formula I and is used for measurement of TT virus gene by a real time detection PCR method and a new reverse side primer comprising an oligonucleotide having a base sequence (in which T may be U) comprising continuous 15-42 bases in a base sequence represented by formula II, used for measurement of TT virus gene by real time detection PCR method. The forward side and the reverse side primers are obtained by comparing, to design and chemically synthesizing base sequences of various TTV strains registered in database.;COPYRIGHT: (C)2000,JPO