目的:研究表皮生长因子受体酪氨酸激酶抑制剂盐酸埃克替尼对体外培养的人舌鳞状细胞癌Tca8113细胞周期的影响.方法:分别应用0、10、20和40μmol/L盐酸埃克替尼处理体外培养的Tca8113细胞24、48和72 h后,采用流式细胞仪分析细胞周期变化.采用间接免疫荧光流式定量检测技术及免疫细胞化学方法检测0、20μmol/L盐酸埃克替尼作用48 h后Tca8113细胞CyclinD1及P27蛋白的表达.结果:随盐酸埃克替尼浓度、作用时间的增加,G0/G1期细胞比例逐渐增加(F浓度=34.791,F时间=19.934,F浓度×时间=9.028,P＜0.001).盐酸埃克替尼作用后Tca8113细胞CyclinD1表达降低,P27表达增加.结论:盐酸埃克替尼通过降低CyclinD1的表达、增加P27的表达,改变Tca8113的细胞周期分布,有明显的G0/G1期阻滞作用.%Aim: To investigate the effect of icotinib on cell cycle of human tongue carcinoma cell line Tca8113. Methods:Tca8113 cells were incubated with icotinib at doses of 0,10, 20, and 40 μ mol/L for 24, 48, and 72 h, respectively. Distribution of cell cycle were detected by flow cytometer( FCM) . Immunocytochemical method and indirectly immu-nfluorescene and FCM were used to detect the expressions of the cell cycle protine CyclinD1 and P27 of Tca8113 cells treated with 0,20 μmol/L icotinib for 48 h. Results: Icotinib induced a remarkable G0/G1 cell cycle arrest in dose-depende-dent and time-dependent manners( Fxoncentrition= 34. 791,Ftime=19.934,Finter= 9.028,P <0. 001) . Icotinib could decreased the expression of cyclinDl and increased P27 expression of Tca8113 cells. Conclusion: Icotinib could make Tca8113 cells arrested at C0/G1 phase through decreasing CyclinDl expression and increasing P27 expression.
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