阿司匹林对人卵巢癌组织和 SKOV3细胞 SOX7表达和 Wnt／β-catenin信号通路的影响

摘要

Aim:To investigate the effect of aspirin on expression of SOX 7 and Wnt/β-catenin signaling pathway in hu-man ovarian cancer SKOV3 cells.Methods:The expression levels of SOX7,β-catenin and Cyclin D1 in ovarian tumor tissue and normal ovarian tissue were detected by quantitative real-time PCR( qRT-PCR) .The SKOV3 cells were stimulated by dif-ferent concentrations of aspirin in vitro and incubated for 48 h, then the expression of SOX 7,β-catenin and Cyclin D1 mRNA were detected by qRT-PCR.The effect of aspirin on SKOV3 cell proliferation was measured by MTT assay .Results:The ex-pression of SOX7 mRNA was decreased in ovarian cancer tissue (t=3.614,P=0.001),but the expressions of β-catenin and Cyclin D1 proteins were increased(t=13.651, 5.967, P<0.001).The expression level of SOX7 mRNA was increased with the treatment of increasing concentration of aspirin (F=12.151,P<0.001),but those of β-catenin mRNA and Cyclin D1 mRNA were decreased(F=6.214,4.903,P<0.05) in SKOV3 cell.Aspirin inhibited the cell proliferation of SKOV3 cells in a concerntration-dependent manner(F=20.481,P<0.001).Conclusion:Aspirin may have effect on Wnt/β-catenin sig-naling pathway by upregulating the expression of SOX 7 mRNA in human ovarian cancer SKOV3 cells.%目的：研究阿司匹林对人卵巢癌组织和SKOV3细胞SOX7表达和Wnt／β-catenin信号通路的影响。方法：采用实时荧光定量PCR技术检测20例卵巢癌组织和20例正常卵巢组织中 SOX7、β-catenin和Cyclin D1 mRNA的表达；0、0．5、1．0、2．0和3．0 mmol／L阿司匹林体外作用SKOV3细胞48 h后，采用实时荧光定量PCR技术检测细胞SOX7、β-catenin和Cyclin D1 mRNA的表达；MTT法检测0、0．5、1．0、2．0和3．0 mmol／L阿司匹林对SKOV3细胞增殖的影响。结果：与正常卵巢组织相比，卵巢癌组织中SOX7 mRNA表达降低（ t＝3．614， P＝0．001），β-catenin和Cyclin D1 mRNA表达升高（t＝13．651和5．967， P＜0．001）。0、0．5、1．0、2．0和3．0 mmol／L阿司匹林作用48 h后，SKOV3细胞中SOX7 mRNA的表达水平逐渐升高（F＝12．151，P＜0．001），而β-catenin和Cyclin D1 mRNA的表达水平逐渐降低（F＝6．214和4．903，P＜0．05）。阿司匹林浓度依赖性地抑制SKOV3细胞的增殖（ F＝20．481，P＜0．001）。结论：阿司匹林可能通过上调人卵巢癌中SOX7的表达对Wnt／β-catenin信号通路产生影响。