离子液体对热带假丝酵母细胞生物相容性的研究

摘要

采用琼脂扩散法、糖代谢活力保留法和细胞生长抑制试验评价了6种离子液体对热带假丝酵母104细胞的毒性大小,以期构建可用于热带假丝酵母细胞催化3,5-双三氟甲基苯乙酮不对称还原的含离子液体反应体系.研究表明:离子液体[EMIM] (L)-Lac对热带假丝酵母细胞的毒性最小,在含该离子液体的体系中进行不对称还原时可获得最高产率.阴离子为(L)-Lac-的离子液体对细胞的毒性较BF4-的低；且阴离子为BF4-时,离子液体对细胞的毒性随阳离子咪唑环上烷基侧链取代基长度的增加而增加.与基于细胞生长抑制试验获得的离子液体EC50值的毒性评价相比较,采用琼脂扩散法和糖代谢活力保留法均可快速、简便地检测离子液体的生物相容性,后两种方法可为快速筛选合适的离子液体种类,以构建含离子液体反应体系提供一定的依据.%The agar diffusion test,glucose metabolic activity retention method and cell growth inhibition test were performed to evaluate the toxicity of ionic liquids to Candida tropicalis 104 cells,and to formulate a suitable ionic liquid-containing system for asymmetric reduction of 3,5bistrifluoromethyl acetophenone.[EMIM](L)-Lac showed less toxicity to Candida tropicalis 104 cells and the maximum yield was obtained when asymmetric reduction carried out in an [EMIM](L)-Lac-containing system.The ionic liquids with (L)-Lac-anion were more biocompatible to Candida tropicalis cells than BF4-.When the anion of ionic liquids was BF4-,an increase chain length of alkyl group substituted on the imidazole cation ring corresponded to an increase in toxicity.Compared with the EC50 value obtained by the cell growth inhibition test,the agar diffusion test and glucose metabolism can be used to fast and simply distinguish between toxic and biocompatible ionic liquids and can be applied for preliminary screening of ionic liquids for constructing of an ionic liquid-containing system for asymmetric reduction.