Biological Compatibility and Cytotoxicity Studies of Hepa-1c1c7, HepG2, and MCF-7 Cancer Cell Lines Upon Exposure to Silver, Palladium, and Gold Nanoparticles.

摘要

The field of nanomedicine has recently explored potential applications for cancer treatment ranging from drug delivery methods to cancer imaging techniques. Due to potential risks of nanomaterials affecting non-cancerous cells in the body, in vitro toxicity studies are being conducted. The nanoparticles (NPs) were synthesized from potassium tetrachloroaurate (III) hydrate, potassium tetrabromoaurate (III) dihydrate, potassium tetrachloropalladate (II), and silver nitrate. NPs were synthesized using the Turkevich method or through the sodium borohydride reduction. Particles synthesized using sodium borohydride were stabilized with cetyltrimethylammonium bromide (CTAB).;The NPs under investigation were used to treat three cancer cell lines. The treated cells were incubated for 24 h and analyzed using the MTT assay to measure proliferation. In addition, the concentrations of each metal engaged with the live cancer cells were determined via acid digestion and ICP-OES quantification of metal uptake. TEM images and XRD patterns show size and morphology upon NP synthesis.