Isolation and Characterization of the Alkane-Inducible NADPH-Cytochrome P-450Oxidoreductase Gene from 'Candida tropicalis'

摘要

The gene coding for the Candida tropicalis NADPH-cytochrome P-450 oxidoreductasewas isolated by immunoscreening of a C. tropicalis (lambda)gt11 expression library and colony hybridization of a C. tropicalis genomic library. The C. tropicalis CPR gene produces a 2.35-kilobase mRNA transcript, levels of which were shown to be increased 16-fold in cells grown on tetradecane relative to cells grown on glucose as the sole carbon source. A 3-kilobase DNA fragment was