浊点萃取-紫外分光光度法同时测定厚朴酚与和厚朴酚的含量

摘要

A new method of cloud point extraction-UV spectrophotometry based on using Triton X-114 as an extractant was developed for simultaneous determination of magnolol and honokiol in traditional Chinese medic -inal material.The effects of the pH of solution , concentrations of surfactant Triton X-114, equilibrium temper-ature and time on the efficiency of cloud point extraction were systematically examined .Under the optimized conditions, the linear equations were Y1 =5.89 ×10 -4x-1.14 ×10 -3(R2 =0.992 1), Y2 =5.52 ×10 -4x+7.71 ×10 -5(R2 =0.999 2), the detection limits were 2.5 μg/L, 1.0 μg/L for magnolol and honokiol, re-spectively .This method was applied to the determination of honokiol and magnolol in traditional Chinese me -dicinal material with recoveries of 98.4%~104.3%.%建立了以Triton X-114为表面活性剂的浊点萃取-分光光度法同时测定厚朴酚与和厚朴酚含量的新方法，探讨了溶液的pH，Triton X-114浓度、平衡温度和平衡时间等因素对浊点萃取的影响．在最佳条件下，厚朴酚与和厚朴酚含量的线性方程分别为 Y1＝5．89×10-4x-1．14×10-3（相关系数为0．9921）， Y2＝5．52×10-4 x＋7．71×10-5（相关系数为0．9992）；检测限分别为2．5μg/L和1．0μg/L．该方法成功用于药材中厚朴酚与和厚朴酚含量的测定，回收率为98．4％～104．3％．