液相微萃取/后萃取-高效液相色谱法测定厚朴及其制剂中厚朴酚与和厚朴酚的含量

摘要

Liquid-phase microextraction with back extraction (LPME-BE) combined with high performance liquid chromatography (HPLC) was investigated for the extraction and determination of magnolol and honokiol in Magnolia officinalis, a traditional Chinese medicine (TCM), and its pharmaceutical preparations, Huo Xiang Zheng Qi peroral liquid and Xiang Sha Yang Wei pellet. Organic solvent, donor and acceptor phases, stirring rate and extraction times were all factors which can influence the efficiency of extraction and were all optimized during the course of this work. Linear calibration curves were obtained in concentration ranges of 1.56-156 μg/mL for magnolol and 1.10-110 μg/mL for honokiol. Detection limits (S/N = 3) were 0.10 and 0.07 μg/mL, respectively. The relative recoveries were both in the range of 98.3%- 105.1% and RSD was lower than 2.5%.%本文采用液相微萃取/后萃取(LPME/BE)与高效液相色谱联用法萃取并测定了中药厚朴及其制剂-藿香正气口服液和香砂养胃丸中厚朴酚与和厚朴酚的含量.优化了萃取效率影响因素,萃取溶剂、供相与接受相、转速及萃取时间.厚朴酚与和厚朴酚的线性范围分别为1.56-156μg/ml和1.10-110μg/mL;检测限(S/N=3)分别为0.10 μg/mL和0.07μg/mL,回收率范围为98.3%-105.1%,RSD<2.5%.