Objective] To observe the antioxidative and hepatoprotective effects of squalene on acute liver injury mice induced by CCl4. [Methods] 60 male ICR mice were randomly divided into 6 groups(n=10). Three groups were orally administered with 0.4g·kg-1, 0.2g·kg-1 and 0.1g·kg-1 dosages of squalene respectively, one group with 1mg·kg-1 dosage of biphenyldicarboxylate, the others(control group and model group) with edible vegetable oil once a day for 30d. At the 29th day, all mice were orally administered with 80mg·kg-1 dosage of CCl4 except the control mice. The levels of ALT and AST in serum were detected, the levels of MDA, GSH and SOD in liver were detected, and the pathology changes of hepatic were observed after 16h fasting at the 31th day. [Results] Compared with the control group, the levels of MDA of model mice are higher(P<0.001), the levels of GSH, SOD of model mice are lower(GSH:P<0.01,SOD:P<0.001), indicating the antioxidation model is effective. Compared with the model group, 0.4g·kg-1 dosage of squalene can raise the levels of GSH(P<0.01) and reduce the levels of MDA(P<0.05) in liver, 0.4g·kg-1 and 0.2g·kg-1 dosage can raise the levels of SOD(P<0.05) in liver. Compared with the control group, the levels of ALT, AST on model mice are higher(P<0.001), and the pathology changes of model mice are significant(P<0.001), indicating the liver injury model is effective. 0.4g·kg-1 dosage of squalene can reduce the serum levels of ALT, AST(ALT:P<0.01, AST:P<0.05), 0.4g·kg-1 dosage can ameliorate the pathology changes of hepatic by CCl4. [Conclusion] Squalene has significant antagonistic effects on GSH, SOD decreasing and MDA increasing in liver tissue of mice induced by carbon tetrachloride. The effects on acute liver injury such as Serum AST, ALT abnormality and liver pathology changes by carbon tetrachloride are also improved. Squalene is of antioxidation and hepatoprotective effects on CCl4 induced acute liver injury in mice. These effects are of dose dependency.%[目的]观察角鲨烯对四氯化碳急性肝损伤模型小鼠的抗氧化和护肝作用。[方法]60只雄性ICR小鼠随机分成6组，分别为角鲨烯0.4g·kg-1、0.2g·kg-1、0.1 g·kg-1组，阳性组，模型组和对照组，每组10只。角鲨烯0.4g·kg-1、0.2g·kg-1、0.1g·kg-1组分别给予角鲨烯0.4g·kg-1、0.2g·kg-1、0.1g·kg-1灌胃，阳性组给予联苯双酯片1mg·kg-1灌胃，模型组和对照组给予等体积的食用植物油灌胃，连续给予30d。第29d除对照组外各组小鼠给予CCl480mg·kg-1灌胃。第31d眼内眦取血（取血前禁食不禁水12h），分离血清，检测血清中谷丙转氨酶（alanine aminotransferase，ALT）和谷草转氨酶（as－partate aminotransferase，AST）水平；取血后处死小鼠，解剖取肝，检测肝组织中的丙二醛（malondialdehyde，MDA）、谷胱甘肽（glutathione，GSH）和超氧化物歧化酶（superoxide dismutase，SOD）的含量及肝组织的病理变化。[结果]与对照组比较，模型组MDA显著增高（P<0.001），GSH、SOD显著降低（GSH：P<0.01，SOD：P<0.001），说明本实验抗氧化动物模型成功，而角鲨烯0.4g·kg-1剂量能显著提高肝组织中的GSH水平（P<0.01），显著降低肝组织中MDA水平（P<0.05）；0.4g·kg-1和0.2g·kg-1能显著提高肝组织中的SOD水平（P<0.05），说明角鲨烯有明显抗氧化作用。与对照组比较，模型组血清ALT、AST均显著升高（P<0.001），肝组织气球样变、脂肪性变性、胞浆凝聚、水样变性及细胞坏死等病理变化的分级积分显著高于对照组（气球样变、脂肪性变性、胞浆凝聚：P<0.001；水样变性、细胞坏死：P<0.01），说明急性肝损伤动物模型成功，而角鲨烯0.4g·kg-1剂量能显著降低血清ALT、AST水平（ALT：P<0.01, AST：P<0.05）；角鲨烯0.4g·kg-1和0.2g·kg-1剂量能显著改善肝细胞的气球样变（0.4g·kg-1：P<0.001,0.2g·kg-1：P<0.01）；角鲨烯0.4g·kg-1剂量能显著改善肝细胞的脂肪性变性、胞浆凝聚、水样变性及细胞坏死等病理改变（P<0.05），说明角鲨烯有明显护肝效果。[结论]角鲨烯对小鼠因四氯化碳引起的肝组织中GSH、SOD下降和MDA升高有显著拮抗作用，对因四氯化碳引起的血清ALT、AST水平升高和肝组织病变等急性肝损伤表现也有显著的改善作用，且有明显的剂量相关性，0.4g·kg-1剂量的角鲨烯与1mg ·kg-1的联苯双酯作用效果相当，表明角鲨烯有较好的抗氧化和护肝作用。
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