血栓调节蛋白在早发型重度子疒间前期患者胎盘组织中的表达

摘要

Objective To explore the relationship between thrombomodulin ( TM ) and early onset severe pre-eclampsia by detecting the level of TM mRNA in placenta tissue in patients with early onset severe preeclampsia .Meth-ods From January 2012 to February 2013 , 60 severe preeclampsia women who deliveried in Xuzhou Maternity and Child Health Care Hospital were recruited in this study , including 30 women with early onset severe preeclampsia ( early onset group) and 30 women with late onset severe preeclampsia (late onset group).23 cases of healthy premature delivery were selected as preterm group and 30 cases of healthy full term pregnancy served as control group ( control group ) .Re-verse transcription-polymerase chain reaction ( RT-PCR) was applied to detect the concentration of TM mRNA in ma-ternal placenta .Results TM mRNA was expressed in placenta of all pregnant women in each group .Significantly lower expression was found in early onset group (P<0.05).Pairwise comparisons of late onset group , preterm group and con-trol group showed no statistically significant difference (P>0.05).Conclusion The early onset and late onset severe preeclampsia may have different pathogenesis .TM may be associated with the pathogenesis of early onset severe pre-eclampsia.%目的：探讨血栓调节蛋白（ thrombomodulin ， TM）在早发型重度子疒间前期患者胎盘组织中的表达及其在发病中的作用。方法选择2012年1月到2013年2月在徐州市妇幼保健院产科住院分娩的重度子疒间前期患者60例，其中，早发型重度子疒间前期患者30例（早发型组），晚发型重度子疒间前期患者30例（晚发型组）；选择同期健康早产产妇23例（早产组），健康足月妊娠产妇30例（对照组）。应用反转录-聚合酶链反应（RT-PCR）检测各组产妇胎盘组织TM mRNA的表达水平。结果 TM mRNA在各组胎盘组织中均有表达，但表达程度有差异。早发型组与早产组、晚发型组、对照组比较差异均有统计学意义（ P＜0．05）；晚发型组与早产组、对照组比较差异均无统计学意义（P＞0．05）。结论早发型与晚发型重度子疒间前期可能存在不同的发病机制，TM可能在早发型重度子疒间前期的病理生理过程中起一定作用。