IL－1β诱导的中枢神经炎症对外周免疫的影响

摘要

目的：研究慢性中枢炎症对大鼠机体健康－外周免疫系统的影响。方法将20只雄性SD大鼠分为2组：对照（ normal saline，NS）组、白细胞介素－1β（ IL－1β）诱导的模型组。大鼠接受侧脑室注射IL－1β14天，高架、旷场实验检测动物的焦虑和应激行为，MTT法测定淋巴细胞和腹腔巨噬细胞的活性，酶联免疫吸附测定法检测血清肿瘤坏死因子（ TNF－α）、过氧化氢（ H2 O2）和糖皮质激素（ GC）的含量。结果与对照比较，IL－1β致使：①大鼠在旷场实验中的自主运动降低，高架实验中的焦虑行为增加（P＜0．05）；②淋巴细胞的增殖能力和巨噬细胞的吞噬功能降低（P＜0．05）；③血清中H2O2和GC的含量（P＜0．05）升高，TNF－α含量降低（P＜0．05）。结论慢性中枢炎症可引起机体外周免疫水平降低和氧化应激损伤。%Objective To investigate the effects of chronic neuroinflammation on the peripheral immune system in rats.Methods A total of 20 male Sprague Dawley ( SD) rats were divided into two groups:a normal saline ( NS) group and an interleukin-1β( IL-1β) induction group where IL-1βwas injected into the lateral ventricle of the rats for 14 days.MTT assay was used to measure the proliferation of lymphocytes and the activity of peritoneal macrophages.The levels of serum tumor necrosis factor ( TNF-α) , hydrogen peroxide ( H2 O2 ) , glucocorticoid ( GC ) were detected by ELISA.Results Compared with the control group, injection of IL-1βcould reduce rats′autonomous mobility in open-field, the proliferation of lymphocytes, the phagocytic function of macrophages and the concentration of TNF-α( P<0.05), while increasing anxiety-like behavior in elevated plus mazes and the levels of H2O2 and GC (P<0.05).Con-clusions Chronic neuroinflammation may suppress the peripheral immune function but enhance oxidative stress.