细胞凋亡相关抗原Fas和Bcl-2 在自身免疫性甲状腺病甲状腺组织中的表达

摘要

Objective To study the expression of Fas and Bcl-2 genes inGraves disease (GD) and Hashimotos disease (HD) and to investigate the relationship between apoptosis and autoimmune thyroid disease (AITD). Methods Thyroid tissues were obtained by core needle biopsy,SABC immunohistochemical methods were used to examine the expression of Fas and Bcl-2 antigen on thyrocytes in situ.Results ①Compared to normal control,the expression of Fas antigen on thyrocytes of both types of AITD patients was increased,the expression of Bcl-2 was also increased in GD, on change of Bcl-2 expression in HD was found.②The expression of Fas antigen on thyrocytes of GD was stronger than that of HD,the expression of Bcl-2 in the former was also stronger than that in the later,Compared to Bcl-2,the intensity of expression of Fas antigen in HD was increased,but there was no significant difference between Fas and Bcl-2 in GD.Conclusion Apoptosis induced by Fas might plays an important role in the pathogenesis of HD,but in GD,the effect of apoptosis induced by Fas might be overcome by Bcl-2.%目的 研究Fas，Bcl-2抗原在Graves病(GD)和桥本氏病(HD)的表达，探讨细胞凋亡在自身免疫性甲状腺病(AITD)发病中的作用。方法 粗针穿刺获得病人甲状腺组织标本，颈部手术时取正常甲状腺组织作为对照；采用SABC免疫组化方法原位观察Fas,Bcl-2抗原的表达。结果 两种AITD甲状腺细胞Fas抗原的表达均较正常对照增强，GDBcl-2抗原表达也较正常对照增强，HDBcl-2表达强度与正常对照无明显差异；GD与HD相比较，GD甲状腺细胞Fas，Bcl-2抗原表达均强于HD。在HD，Fas抗原表达较Bcl-2表达明显增强，但在GD，Fas抗原表达与Bcl-2表达强度无显著差异。结论 由Fas介导的细胞凋亡在HD发病中起重要作用，在GD，Fas诱导细胞凋亡的作用可能被Bcl-2对抗。