目的 探讨Forkhead box M1对肺癌细胞侵袭能力的影响,为肺癌的基因靶向治疗提供理论依据.方法 应用RNA干扰技术下调FoxM1在肺癌细胞株A549中的表达,观察肺癌细胞侵袭能力的改变,并检测侵袭相关蛋白MMP-2的表达情况.结果 运用RNA干扰技术能够有效下调FoxM1基因及蛋白表达水平,下调FoxM1表达水平降低了肺癌细胞的侵袭能力,并引起了肺癌细胞中MMP-2的表达下降.结论 下调FoxM1表达水平能够抑制肺癌细胞的侵袭能力,提示FoxM1可能成为肺癌治疗的新靶点.%Objective To explore the effects of small interfering RNA (siRNA) knockdown of forkhead box M1 (FoxM1) on the invasiveness of human lung cancer cell A549 in vitro so as to provide theoretical evidence for gene-targeted therapy for lung cancer. Methods FoxM1 siRNA was transfected into human lung cancer A549 cells with lipofectamine 2000. Protein expression level was evaluated by Western blotting. Anchorage-independent growth and invasiveness of A549 cells were measured by soft agar colony formation and transwell cell invasion assay, respectively. Results FoxM1 siRNA was successfully transfected into A549 cells, resulting in the significant inhibition of FoxM1 mRNA and protein expression. Down-regulation of FoxM1 decreased the invasiveness of A549 cells and reduced the expression of matrix metalloproteinase-Z (MMP-2) of lung cancer cells. Conclusion Down-regulation of FoxM1 can inhibit the invasiveness of human lung cancer cells, suggesting that FoxM1 is a potential target for lung cancer therapy.
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