基质金属蛋白酶-9、基质金属蛋白酶组织抑制剂-1测定在慢性肾衰竭患者中的变化

摘要

AIM:To investigate the effect of matrix metallopro?teinase?9 ( MMP?9 ) and tissue inhibitor of tissue inhibitor of matrix metalloproteinase?9 ( TIMP?1) in the pathological changes of chronic renal failure by measuring the expression of MMP?9 and TIMP?1 in patients with chronic renal failure. METHODS: The concentration of MMP?9 and TIMP?1 in serum was determined by ELISA method in patients with different stages of chronic renal failure. RESULTS:The serum level of MMP?9 had no significant differences among different groups ( P>0. 05 ) , the serum and urine level of TIMP?1 had an ascending tendency with the severity of renal failure, and there were significant differences among different groups (P<0.05). All index except α1?MG and β2?MG in patients with chronic renal failure were significantly higher than those of control group (P<0.05). CONCLUSION: Through the detection of serum MMP?9, TIMP?1 and urine TIMP?1 concentra?tions, we can better understand the degree of chronic renal failure and the formation of renal interstitial fibrosis.%目的：测定基质金属蛋白酶（ MMPs）和基质金属蛋白酶组织抑制剂（ TIMPs）在慢性肾衰竭患者中的结果，以期了解这两种物质在慢性肾衰竭病变中的作用．方法：选择不同期的慢性肾衰竭患者作为研究对象，采用ELISA法测定血清中的MMP?9和TIMP?1的浓度．结果：血清中MMP?9虽然在CRF病例中较高但方差分析提示，差异无统计学意义（ P＞0．05）；血清TIMP?1的结果按肾功能衰竭的程度呈上升趋势，以CRFⅣ组的结果为最高，CRFⅠ组最低，组间比较差异有统计学意义（ P＜0．001）；尿液中TIMP?1的结果以CRFⅣ组的结果为最高，CRFⅠ组最低，组间比较差异有统计学意义（ P＜0．001）．慢性肾衰竭患者除CRFⅠ组α1?MG、β2?MG指标外，其余指标均显著高于对照组，差异有统计学意义（P＜0．05）．结论：通过检测血清MMP?9、TIMP?1以及尿液TIMP?1的浓度可以更好地了解慢性肾衰竭的程度和肾间质纤维化的形成原因．